What Is Blood Smear Procedure For Nurses And Doctors

What is blood smear procedure.Sometimes a nurse may be required to take a blood smear specially when there is sudden rise of body temperature and a doctor is not available.

Preparation of thin blood smear

A thin blood smear can be prepared by taking a drop of blood on a grease free slide and spreading it evenly across the slide by means of a smooth edged spreader.

What You Should Know About Blood smear Procedure

  • Select a grease free slide duly cleaned.
  • Select another spreader slide having smooth edge
  • Prick the finger or ear lobule or heel as the case’may be and collect a drop of blood at one end (rt. end) on the slide. For collection of a drop of blood various approach can be applied. You can touch the pricked finger with blood on to the slide. Or you can touch the slide at rt. end to the finger. Or even you can take away the drop of blood by the spreader slide and make contact with smear slide ar 30° angle. When it spreads at the contact edge of t spreader, slide over the smear slide.
  • If the drop of blood is collected either by touching smear ” slide or finger, also keep contact the spreader s!i at angle of 30° and wait it to spread at the breadth spreader slide.
  • For RBC morphology or differential count a thin smear required. So far achieving this, move spreader slide from
  • right to left quickly in a steady uniform motion to get good thin smear. A well spread smear shows no lines extending across or downwards through the film and the smear should be tongue shaped.

Making Thick Smear Blood

Thick blood smear are required for detection of malaria parasite and microfilariae. Here a large drop of blood is taken at the centre of the slide not at the end like thin smear. Thereby means of a needle or corner of a slide, the drop is spread over a area of 1/2 inch square on the slide. To have correct thickness achieved, after drying the smear, printed letter should be seen through the thickness of the smear.


Usually blood is collected from vein over arm for various lab tests, besides pricking the finger.


  • Intimate the procedure to the patient.
  • Reassure the patient as some nervous and sensitive patients may go into vaso-vagal shock during puncture into vein.
  • Take all sterile equipments and adopt all sterile procedure as for I.V. technique.
  • Inspect the vein or use a tourniquet if required.
  • Use syringe depending on the amount of blood required.
  • Needle should be 22 size or less.
  • Ask the patient to close and open his first, several times to allow return of more venous blood.
  • Puncture the vein as I.V. technique and withdraw piston of syringe to take out blood in syringe. Take the amount re­quired.
  • Ask the patient to open his first.
  • Transfer the blood from the syringe to the container after removing the needle and through the nozzle of the syringe and not through the needle.

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