How To Maximize Efficiency For The Development And Validation Of Methods?

As we know the search for faster , selective and sensitive methods of analysis is one of the crucial objectives pursued by each analysis laboratory. That is why today I want to talk a little about the development of chromatographic methods and a way to maximize their efficiency.

Developing an analytical method aims to identify and determine its purity (for an active substance), and quantify it univocally (for a specific substance present in an environmental sample or in a mixture). Development work includes different staff members and can be done in various ways. On the one hand, it may involve adapting an existing method by making minor changes to adapt it to a new application. And, on the other hand, the analyst can begin to sketch some ideas and apply his knowledge and experience to devise a suitable method involving, of course, a greater effort, in addition to knowing how ideal the final method is.

Despite the effort invested in the development of the method, there is no guarantee that the method will work properly during its validation, or under routine conditions in a particular laboratory. By involving different personnel in the development and validation phase we can verify, by means of the instructions, that this is understandable and applicable, ensuring that it is appropriate for the analysis in question and that the results obtained are reliable and consistent.

However, when we talk about an industrial or research analysis, the speed of the quantitative methodological process is an important key since it usually conditions the characteristics of the method used, rather than its sensitivity, which, in most analytical methods is the need to determine substances in very small quantities. With this in mind, the question arises, how can I maximize efficiency during the development of my analytical methods?

The solution I present to you today is Shimadzu’s Method Scouting System , a system that will help you improve efficiency in the development of liquid chromatography analysis methods . By means of a control software and a robust and high pressure resistant column switching system to achieve a fast workflow for method exploration.

How does it work? The explanation I will give below will be with Shimadzu chromatographic equipment , if you have them and want to know more about this software, please contact us with pleasure we will give you a free advice. If you do not have Shimadzu equipment and instrumentation and want to know more about the brand, also contact us , we will gladly answer all your questions. Without further saying continuous with the improvement system for the development of your methods.

Method exploration can now be done with Shimadzu’s diverse line of LC products . Choose the right system for your laboratory environment, whether for CFS investigations or simple investigations in compact systems.

Nexera Scouting Method

  • Capable of automated investigation of conditions in the range of 130 MPa, including 192 patterns of 8 mobile phases and 12 columns.
  • It is also able to investigate pH conditions with mobile phase mixing.

Nexera / UCs supercritical fluid chromatography switching system UHPLC / SFC

  • Optimized for the method of investigating chiral compounds.
  • It is also compatible with LC / SFC switching, for even greater efficiency improvements in method development.

Exploration of Series I Methods

  • Optimized for the development of simple methods with a single pump.
  • Low cost design and space saving.


by Abdullah Sam
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