1.- Obtain an experimental tumor strain through the transplantation of primary chemo-induced tumors
2.- Specify the latency of the tumor and the survival of the new receptors.
3.- Characterize the tumor’s response to monochemotherapy according to its volumetric growth.
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4.- Evaluate the immunogenic character of the tumor and its behavior in parental
5.- Identify the radiosensitivity of the tumor according to its volumetric regression.
6.- Evaluate the effect of treatment with recombinant human epidermal growth factor (EGFh-r) on the survival of transplanted animals
Materials and methods
General Methods Used in Different Biological Tests
The IBF1 mice were selected, in which the induction of squamous cell carcinomas was achieved with the topical application of Cigarette Smoke Condensate, according to established method (35). See illustrations that guide the different morphological stages of the carcinogenesis process prior to the “in vivo” transplant cycle. The tumors were surgically resected under sterile conditions under laminar flow prior to aseptic and lethal anesthesia of the animals.
Once the samples were taken for morphological studies, fragments in saline solution were inoculated to the first receptors through a hypodermic needle No. 18. The remaining serial passes were made by means of a suspension of tumor cells obtained by means of a tissue homogenizer, adjusting it volumetrically with physiological saline solution at a 1: 2 ratio. the historical characteristics of it.
In each animal the transplant was performed by depositing a 0.2 ml subcutaneous inoculum of the tumor suspension described above in the axillary region.
Once the tumor latency time was expected, the experimental groups were formed for the different studies according to the description of the methods and procedures recommended by the WHO for toxicity bioassays (36).
To assess the growth of the tumors, their volume was determined. For this, the two main diameters were measured and replaced in the following formula (37):
V = 4/3 (R1 + R2) / 2
Where R1 and R2 are the radii obtained from the perpendicular measurements of the tumor.
Tumor growth inhibition (ICT) was calculated according to formula (37):
ICT = V treated – V control ……. x 100
Where V is the average volume of tumors in the treated and control animal groups.