The method of conducting pheromone monitoring of the eastern moth

To monitor the eastern codling moth, deltoid traps with an adhesive insert are most often used. Collected pheromone traps are placed in the surveyed areas by shuttle method at the rate of 1 pc. on 5 hectares. They are placed in the crowns of trees at an altitude of 1.5-2 m, to identify new foci – from the second decade of July to the third decade of September to monitor the dynamics of flight and forecasting from April to October. The rate of trap placement per inspector per day is 15–20, per week – 75–100.

After placing the traps stationary every seven days in the same manner spend sampling butterflies and replacing the liners. If a rubber dispenser with pheromone is used in the trap, then its tweezers are transferred to a new liner.

All traps and their inserts must be numbered, a diagram of their placement in the garden indicating the location of each. Adhesive inserts for each sampling date are accompanied by a layout diagram. On the inserts indicate the number of the trap, the date of installation and sampling, the name of the inspector, after which they are sent for analysis to the laboratory.

In laboratory conditions, the abdomen of all butterflies is separated from one insert and placed together in one cell of the cuvette (you can use cuvettes for germinating seeds). On each insert, the cell number (1A, 2A, etc.) is recorded in which the butterflies are placed. After filling all the cells with butterflies from each insert for one sampling date, they are filled with potassium alkali (10%) or lactic acid (40%) and left for a day. The next day, they look at the contents of each cell separately on the glass with the hole and determine the types of butterflies by their genitals (Fig. 32), which are carefully separated by two dissecting needles under the binocular with backlight. After separating the genitals and establishing the accessory of the butterflies, they are counted by type, the numbers are written on the back of the insert or in the corresponding card, journal. If genitalia of the eastern moth are discovered, they are placed separately in a test tube with a label indicating the location, date of detection, and sent to a quarantine laboratory to confirm the results. Genitalia by type can be stored in small test tubes with labels in a solution of glycerol with alcohol (1: 1). Genital leaflet types are established in accordance with the “Identifier of Insects of the European Part of the USSR” (T. IV. Part 1. 1978. Edited by G. S. Medvedev) or later reference publications.

After the inspection, each farm draws up an act which indicates the area of ​​plantations, the number of traps used, samples, caught butterflies, including the eastern moth (after confirmation by the quarantine laboratory, the number and date of the examination certificate are indicated). The act is signed by the representative of the farm, inter-district inspector and surveyor. Based on the survey results, appropriate quarantine measures are developed to localize and eliminate the outbreak, taking into account the number of pests found in traps.

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